Generator
Part:BBa_K1809024:Design
Designed by: David Morgan Group: iGEM15_WPI-Worcester (2015-08-26)
Const. Promoter-RBS-BrAFP-DT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 389
Illegal AgeI site found at 163
Illegal AgeI site found at 418 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used a strong promoter to produce high levels of expression of BrAFP.
Source
Composite of parts BBa_J23119 and BBa_K1809023